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bca quantitative detection kit  (Vazyme Biotech Co)


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    Vazyme Biotech Co bca quantitative detection kit
    Bca Quantitative Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1436 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bca+quantitative+kit/BCA+Protein+Quantification+Kit/pmc12887172-62-7-11
    Average 97 stars, based on 1436 article reviews
    bca quantitative detection kit - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Lysis:

    Article Title: Transdermal Delivery of Resveratrol by a Nanomicelle-Loaded Enzyme-Triggered Hydrogel for Diabetic Wound Healing
    Article Snippet: Wound infections can lead to serious diabetes complications that are difficult to cure.. Despite the great efforts made, exploration of bioactive patches to effectively promote diabetic wound healing remains a challenge.. Here, we propose an enzymetriggered self-assembly oligopeptide hydrogel-embedded cell-penetrating peptide modified on the surface of a resveratrol-loaded nanomicelle (Res-CNM/Gel) to enhance the delivery of resveratrol for wound healing.

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Protein Concentration:

    Article Title: Integrative analysis of circRNA, miRNA, and mRNA profiles to reveal ceRNA regulation in geese ovarian development from the birth /laying to ceased periods
    Article Snippet: .. The protein concentration was determined by the BCA quantitative detection kit (Vazyme, Nanjing, China). .. The operation was carried out in accordance with the SDS-PAGE and Western blot kit instructions (Abcam plc, Cambridge, UK).

    Article Title: Screening and Functional Annotation of Differentially Expressed Genes in Ovarian Tissue of Wanxi White Geese during Different Oviposition Phases
    Article Snippet: .. The protein concentration was determined by the BCA quantitative detection kit (Vazyme, Nanj ing, China). .. The operation was carried out in accordance with the SDS-PAGE and Western blot kit instructions (Abcam plc, Cambridge, UK).

    Article Title: Integrative analysis of circRNA, miRNA, and mRNA profiles to reveal ceRNA regulation in geese ovarian development from the birth /laying to ceased periods
    Article Snippet: .. The protein concentration was determined by the BCA quantitative detection kit (Vazyme, Nanjing, China). .. The operation was carried out in accordance with the SDS-PAGE and Western blot kit instructions (Abcam plc, Cambridge, UK).

    Quantitative RT-PCR:

    Article Title: l-theanine alleviates ulcerative colitis by repairing the intestinal barrier through regulating the gut microbiota and associated short-chain fatty acids.
    Article Snippet: Ulcerative colitis (UC) is closely related to impaired intestinal barrier function and imbalanced gut microbial communities.. L-theanine shows great potential in maintaining intestinal integrity and regulating the gut microbiota and associated short-chain fatty acids (SCFAs).. However, whether L-theanine can alleviate UC by repairing the intestinal barrier through these regulatory processes remains unclear.

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Fluorescence:

    Article Title: l-theanine alleviates ulcerative colitis by repairing the intestinal barrier through regulating the gut microbiota and associated short-chain fatty acids.
    Article Snippet: Ulcerative colitis (UC) is closely related to impaired intestinal barrier function and imbalanced gut microbial communities.. L-theanine shows great potential in maintaining intestinal integrity and regulating the gut microbiota and associated short-chain fatty acids (SCFAs).. However, whether L-theanine can alleviate UC by repairing the intestinal barrier through these regulatory processes remains unclear.

    Virus:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Isolation:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Recombinant:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    In Vitro:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Transfection:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Western Blot:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Real-time Polymerase Chain Reaction:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Bicinchoninic Acid Protein Assay:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).

    Software:

    Article Title: SaaS sRNA promotes the interfering effect of Salmonella on hepatic iron metabolism via modulating ferroportin 1.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho-Smad1(Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) Cell Signaling Technology Cat#13820; RRID:AB_2493181 Phospho-STAT3 (Tyr705) Abcam Cat#ab32143; RRID:AB_2286742 GPX4 Abcam Cat#ab125066; RRID:AB_10973901 GAPDH Thermo Pierce Cat#ab9485; RRID:AB_307275 Goat anti-Mouse IgG (H + L) Secondary antibody Thermo Pierce Cat#31210; RRID:AB_228334 Bacterial and virus strains Salmonella Enteritidis: WT Isolated from contact surfaces of meat processing equipment (Wang et al.49) NCM61 Salmonella Enteritidis: DsaaS Deleting saaS gene based on WT (Wang et al.49) NCM282 Salmonella Enteritidis: DsaaS/psaaS Completing saaS gene based on DsaaS/psaaS (Wang et al.49) N/A Chemicals, peptides, and recombinant proteins Luria-Bertani broth HopeBio HB0128 DMEM HyClone SH30022.01B Hieff TransTM in vitro siRNA/miRNA Transfection Reagent Yeasen 40806ES02 Cell lysis buffer for Western and IP Beyotime P0013 PrimeScriptTM RT Master Mix Takara RR036 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711 Critical commercial assays FastPure Cell/Tissue Total RNA Isolation Kit Vazyme RC101 BCA protein assay kit Thermo Scientific 23225 Mouse Ferroportin1 (FPN1) ELISA Kit Ruidahenghui RD-RX28086 Total Superoxide Dismutase Assay Kit with WST-8 Beyotime S0101S Experimental models: Cell lines Human: HepG2 cells Cell Bank of the Chinese Academy of Sciences SCSP-510 Experimental models: Organisms/strains Mouse: BALB/c Ziyuan Laboratory Animal Technology Company N/A Oligonucleotides Primers for strain construction, see Table S1 This paper N/A Primers for RT-qPCR analysis and sequences of siRNA duplexes, see Table S2 This paper N/A Recombinant DNA Plasmids for strain construction, See Table S1 This paper N/A Software and algorithms ImageJ National Institutes of Health, USA https://imagej.nih.gov/ij/ SAS SAS Institute Inc https://www.sas.com Prism GraphPad https://www.graphpad.com/ scientificsoftware/prism/ e1 iScience 28, 111660, February 21, 2025 .. Six to seven-week-old female BALB/c mice (Specified pathogen-free, SPF) were provided by Ziyuan Laboratory Animal Technology Company (Wuhan, China).



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